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Proteintech wnt5a b
Wnt5a B, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 117 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/55184+1+ap/WNT5A%2FB+Antibody/pm41906249-54-8-37
Average 96 stars, based on 117 article reviews
wnt5a b - by Bioz Stars, 2026-10
96/100 stars

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Article Title: SEL1L-HRD1 ER-associated degradation suppresses hepatocyte hyperproliferation and liver cancer
Article Snippet: WNT5A , Proteintech , 55184-1-AP.



Chromatin Immunoprecipitation:

Article Title: Cytoneme-mediated transport of active Wnt5b-Ror2 complexes in zebrafish.
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used WNT5A-B, rabbit PolyAb, ProteinTech, 55184-1-AP, 1:50; ROR2 (D3B6F), rabbit mAb, Cell Signalling Technology, 88639S, 1:50; antirabbit antibody Alexafluor 488, ab150077, Abcam, 1:1000; donkey anti-goat antibody AlexaFluor 647, ab150135, Abcam, 1:1000 Validation All primary antibodies have been validates by IHC and WB as stated in the manuscript. ..

Flow Cytometry:

Article Title: Cytoneme-mediated transport of active Wnt5b-Ror2 complexes in zebrafish.
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used WNT5A-B, rabbit PolyAb, ProteinTech, 55184-1-AP, 1:50; ROR2 (D3B6F), rabbit mAb, Cell Signalling Technology, 88639S, 1:50; antirabbit antibody Alexafluor 488, ab150077, Abcam, 1:1000; donkey anti-goat antibody AlexaFluor 647, ab150135, Abcam, 1:1000 Validation All primary antibodies have been validates by IHC and WB as stated in the manuscript. ..

Magnetic Resonance Imaging:

Article Title: Cytoneme-mediated transport of active Wnt5b-Ror2 complexes in zebrafish.
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used WNT5A-B, rabbit PolyAb, ProteinTech, 55184-1-AP, 1:50; ROR2 (D3B6F), rabbit mAb, Cell Signalling Technology, 88639S, 1:50; antirabbit antibody Alexafluor 488, ab150077, Abcam, 1:1000; donkey anti-goat antibody AlexaFluor 647, ab150135, Abcam, 1:1000 Validation All primary antibodies have been validates by IHC and WB as stated in the manuscript. ..

Biomarker Discovery:

Article Title: Cytoneme-mediated transport of active Wnt5b-Ror2 complexes in zebrafish.
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used WNT5A-B, rabbit PolyAb, ProteinTech, 55184-1-AP, 1:50; ROR2 (D3B6F), rabbit mAb, Cell Signalling Technology, 88639S, 1:50; antirabbit antibody Alexafluor 488, ab150077, Abcam, 1:1000; donkey anti-goat antibody AlexaFluor 647, ab150135, Abcam, 1:1000 Validation All primary antibodies have been validates by IHC and WB as stated in the manuscript. ..

Immunohistochemistry:

Article Title: Cytoneme-mediated transport of active Wnt5b-Ror2 complexes in zebrafish.
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used WNT5A-B, rabbit PolyAb, ProteinTech, 55184-1-AP, 1:50; ROR2 (D3B6F), rabbit mAb, Cell Signalling Technology, 88639S, 1:50; antirabbit antibody Alexafluor 488, ab150077, Abcam, 1:1000; donkey anti-goat antibody AlexaFluor 647, ab150135, Abcam, 1:1000 Validation All primary antibodies have been validates by IHC and WB as stated in the manuscript. ..

Western Blot:

Article Title: Cytoneme-mediated transport of active Wnt5b-Ror2 complexes in zebrafish.
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used WNT5A-B, rabbit PolyAb, ProteinTech, 55184-1-AP, 1:50; ROR2 (D3B6F), rabbit mAb, Cell Signalling Technology, 88639S, 1:50; antirabbit antibody Alexafluor 488, ab150077, Abcam, 1:1000; donkey anti-goat antibody AlexaFluor 647, ab150135, Abcam, 1:1000 Validation All primary antibodies have been validates by IHC and WB as stated in the manuscript. ..

Incubation:

Article Title: Mechanistic study of miR-369-3p in regulating the Wnt/β-catenin signaling pathway via targeting SPTBN1 in inflammatory response and bone destruction of rheumatoid arthritis
Article Snippet: Proteins were transferred onto PVDF membranes (MilliporeSigma; cat. no. IPVH00010) that had been pre-activated in methanol, using a current of 300 mA. .. Membranes were blocked with 5% non-fat milk at room temperature for 2 h and subsequently incubated overnight at 4°C with primary antibodies: β-actin (1:1,000; Zs-BIO; cat. no. TA-09), SPTBN1 (1:500) (HUABIO, HA500014 ), Wnt5 (1:1,000) (Proteintech, 55184-1-AP) and β-catenin (1:500) (Affinity, AF6266). .. After washing, membranes were incubated with HRP-conjugated secondary antibodies (1:10,000) (Zs-BIO, ZB-2301) for 2 h. Protein bands were visualized using enhanced chemiluminescence reagent (GLPBIO; cat. no. GK10008).

Article Title: Mechanistic study of miR‑369‑3p in regulating the Wnt/β‑catenin signaling pathway via targeting SPTBN1 in inflammatory response and bone destruction of rheumatoid arthritis.
Article Snippet: Proteins were transferred onto PVDF membranes (MilliporeSigma; cat. no. IPVH00010) that had been pre‐acti‐ vated in methanol, using a current of 300 mA. .. Membranes were blocked with 5% non‐fat milk at room temperature for 2 h and subsequently incubated overnight at 4 ̊C with primary antibodies: β‐actin (1:1,000; Zs‐BIO; cat. no. TA‐09), SPTBN1 (1:500) (HUABIO, HA500014), Wnt5 (1:1,000) (Proteintech, 55184‐1‐AP) and β‐catenin (1:500) (Affinity, AF6266). .. After washing, membranes were incubated with HRP‐conjugated secondary antibodies (1:10,000) (Zs‐BIO, ZB‐2301) for 2 h. Protein bands were visualized using enhanced chemilumines‐ cence reagent (GLPBIO; cat. no. GK10008).



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Experimental validation of bioinformatically identified inflammatory cytokines, ECM-related, and calcification-regulatory genes. (A) Heatmap illustrating genes involved in regulating osteoblast growth. (B) PPI network of calcification-regulatory genes from BP terms, highlighting hub genes such as Igf1, Tgfb3, and MMP-9. (C) PPI network of ECM-regulatory genes derived from CC terms, highlighting hub genes such as Igf1, <t>Wnt5a,</t> and Spp1. (D) Western blot analysis and quantitative analysis of COX-2/β-actin, MMP-9/β-actin, Wnt5a/β-actin, Spp1/β-actin, Tgfb3/β-actin and Igf1/β-actin ratios in thrombin- and PD03-treated osteoblasts. (E) The ratios of COX-2/β-actin, MMP-9/β-actin, Wnt5a/β-actin, Spp1/β-actin, Tgfb3/β-actin and Igf1/β-actin were compared among different groups. Data are presented as mean ± SD (n = 3). P-values were determined by one-way ANOVA (multi-group comparisons) (*p < 0.05; **p < 0.01; ***p < 0.001; ns, P >0.05). Scale bar: 100 μm.
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Experimental validation of bioinformatically identified inflammatory cytokines, ECM-related, and calcification-regulatory genes. (A) Heatmap illustrating genes involved in regulating osteoblast growth. (B) PPI network of calcification-regulatory genes from BP terms, highlighting hub genes such as Igf1, Tgfb3, and MMP-9. (C) PPI network of ECM-regulatory genes derived from CC terms, highlighting hub genes such as Igf1, <t>Wnt5a,</t> and Spp1. (D) Western blot analysis and quantitative analysis of COX-2/β-actin, MMP-9/β-actin, Wnt5a/β-actin, Spp1/β-actin, Tgfb3/β-actin and Igf1/β-actin ratios in thrombin- and PD03-treated osteoblasts. (E) The ratios of COX-2/β-actin, MMP-9/β-actin, Wnt5a/β-actin, Spp1/β-actin, Tgfb3/β-actin and Igf1/β-actin were compared among different groups. Data are presented as mean ± SD (n = 3). P-values were determined by one-way ANOVA (multi-group comparisons) (*p < 0.05; **p < 0.01; ***p < 0.001; ns, P >0.05). Scale bar: 100 μm.
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Image Search Results


Experimental validation of bioinformatically identified inflammatory cytokines, ECM-related, and calcification-regulatory genes. (A) Heatmap illustrating genes involved in regulating osteoblast growth. (B) PPI network of calcification-regulatory genes from BP terms, highlighting hub genes such as Igf1, Tgfb3, and MMP-9. (C) PPI network of ECM-regulatory genes derived from CC terms, highlighting hub genes such as Igf1, Wnt5a, and Spp1. (D) Western blot analysis and quantitative analysis of COX-2/β-actin, MMP-9/β-actin, Wnt5a/β-actin, Spp1/β-actin, Tgfb3/β-actin and Igf1/β-actin ratios in thrombin- and PD03-treated osteoblasts. (E) The ratios of COX-2/β-actin, MMP-9/β-actin, Wnt5a/β-actin, Spp1/β-actin, Tgfb3/β-actin and Igf1/β-actin were compared among different groups. Data are presented as mean ± SD (n = 3). P-values were determined by one-way ANOVA (multi-group comparisons) (*p < 0.05; **p < 0.01; ***p < 0.001; ns, P >0.05). Scale bar: 100 μm.

Journal: Frontiers in Immunology

Article Title: PD0325901 alleviates thrombin-inhibited osteogenic differentiation through an IL-1β-activated feedback loop between MEK-Erk1/2 and NF-κB signal pathways: insights from bioinformatics and experimental verification

doi: 10.3389/fimmu.2026.1730337

Figure Lengend Snippet: Experimental validation of bioinformatically identified inflammatory cytokines, ECM-related, and calcification-regulatory genes. (A) Heatmap illustrating genes involved in regulating osteoblast growth. (B) PPI network of calcification-regulatory genes from BP terms, highlighting hub genes such as Igf1, Tgfb3, and MMP-9. (C) PPI network of ECM-regulatory genes derived from CC terms, highlighting hub genes such as Igf1, Wnt5a, and Spp1. (D) Western blot analysis and quantitative analysis of COX-2/β-actin, MMP-9/β-actin, Wnt5a/β-actin, Spp1/β-actin, Tgfb3/β-actin and Igf1/β-actin ratios in thrombin- and PD03-treated osteoblasts. (E) The ratios of COX-2/β-actin, MMP-9/β-actin, Wnt5a/β-actin, Spp1/β-actin, Tgfb3/β-actin and Igf1/β-actin were compared among different groups. Data are presented as mean ± SD (n = 3). P-values were determined by one-way ANOVA (multi-group comparisons) (*p < 0.05; **p < 0.01; ***p < 0.001; ns, P >0.05). Scale bar: 100 μm.

Article Snippet: The following antibodies were used in this study: β-actin (AF5001, Beyotime, China), Col1α1 (ab270993, Abcam, UK), Runx2 (AF2593, Beyotime, China), OCN (AF6297, Beyotime, China), Igf1 (AF7179, Beyotime, China), Wnt5a (29793-1-AP, Proteintech, USA), Tgfb3 (AF8142, Beyotime, China), Spp1 (A5427, Bimake, USA), COX-2 (F0327, Bimake, USA), Matrix Metalloproteinase-9 (MMP-9, AF5234, Beyotime, China), Minichromosome Maintenance Complex Component 2 (MCM2, A5172, Bimake, USA), Proliferating Cell Nuclear Antigen (PCNA, SC-25280, Santa, USA), PAR-1 (AF6837, Beyotime, China), IL-1RA (AF7218, Beyotime, China), p65 (8242S, CST, USA), p-p65 (3033T, CST, USA), Erk1/2 (4695T, CST, USA), p-Erk1/2 (4370S, CST, USA), p-Stat3 (Y705) (9145S, CST, USA), p-Stat3 (S727) (9134S, CST, USA), and Stat3 (4904T, CST, USA).

Techniques: Biomarker Discovery, Derivative Assay, Western Blot